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Characteristics and functions:
LSM 710 NLO system is versatile and capable of dealing with extensive
range of research tasks, it is ideal for
highly sensitive fluorescent studies on living depth
samples or organisms. Precision installation of a femtosecond laser
and maximizing detection provides high-quality
image in the deeper layers of tissue. Experiments on photo-and blichingu
photo manipulation can be performed with great accuracy thanks to
given 3D excitation zone. The following applications: time
a series of images, multi-fluorescence image and
measurement of ion concentrations, in transmitted light microscopy, FRET analysis.
The scanning module LSM 710
- Scanning module with two single-channel high-sensitivity
and detectors with 34-channel spectral detector for fast
Parallel capture the full emission profile;
- Random selection of the spectral range of the signal was recorded:
resolution up to 3 nm (sequential scan) and 10 nm
(Parallel scanning);
- Detection of the transmitted light;
- Two independent scanning galvanometer mirror;
- The scanning resolution of 4 x 1 x 6144 to 6144 pixels;
- 14 x 2 scan speeds, 5 frames / sec at 512 x 512
pixels, 0.38 msec / line of 512 pixels (2619 lines / sec);
- Scanning ZOOM increase from 0.6h to 0.1h 40x with pitch;
- The free rotation of 360 ° at the scanning frame;
- Motorized confocal pinhole modulating
diameter and position;
- Bit data 8, 12 or 16 bits.
The laser module LSM 710
- AOTF - stabilized acousto-temperature
continuously variable filter, simultaneous control of the intensity to
6 laser lines, switching time <5 sec;
- Lasers with pre-, pre-aligned
optical fiber (Pigtail-coupled);
The visible range (VIS): Ar-laser (458, 488, 514 nm) 25 mW;
DPSS-laser (561 nm) 20 mW; HeNe-laser (633 nm) 5 mW;
- Up to 6 laser sources simultaneously.
Combination of the LSM 710 with a short-pulse femtosecond infrared
laser with a tunable range (800 - 1500nm) for
multiphoton excitation of fluorescence. Multiphoton process
excitation of the dye (Simultaneous Absorption of several
low-energy infrared photons) based on the effects of
nonlinear optics (NLO) and is expressed in the shorter-wavelength emission
fluorophore.
This approach has several main positive points:
a) higher, by 6-7 times, the sensitivity of the system, as in this
if (fluorophore excitation is possible only within the focal
microvolume, less than 0.1 mkm3) fluorescence, without the need for confocal
point grid, displayed directly on the outer more
highly NDD-detectors;
b) A large, up to 500 microns, the depth of penetration into living tissues and organs,
opportunity to work with whole physiological objects, by virtue of
weak light scattering of infrared radiation;
a) undamaged long, up to several hours, monitoring
objects, due to the low cytotoxicity of infrared radiation and
reduce cellular damage photoinduced processes;
d) Precision Z-localized bleaching (FRAP-technique) of the object
for removal of the kinetic characteristics of transport of molecules in
compartments of cells in the absence of excitation and bleaching
fluorochromes outside the focal microvolume.
The above mentioned features the LSM 710 NLO are unique and
indispensable tool for research complex
physiological processes in living objects macrobiotic. |
